A small, synthetic peptide for gene delivery via the serpin–enzyme complex receptor
Identifieur interne : 002421 ( Main/Exploration ); précédent : 002420; suivant : 002422A small, synthetic peptide for gene delivery via the serpin–enzyme complex receptor
Auteurs : Sheetal Patel [Royaume-Uni] ; Xiaohong Zhang [Royaume-Uni] ; Louise Collins [Royaume-Uni] ; John W. Fabre [Royaume-Uni]Source :
- The Journal of Gene Medicine [ 1099-498X ] ; 2001-05.
English descriptors
- Teeft :
- Amino, Amino acid peptide, Amino acids, Average diameter, Bifunctional, Chloroquine, Complex receptor, Control peptide, Copyright, Culture medium, Dmem, Dmem medium, Effective complexes, Fvflm motif, Gene, Gene delivery, Gene transfer, Huh7, Huh7 cell line, Huh7 cells, Irrelevant peptide, Isotonic solutions, John wiley sons, Ligand, Lysine, Moiety, Nacl, Pat1, Pat1 peptide, Pat2, Pat2 peptide, Pat2 vector, Peptide, Pgl2, Pgl2 plasmid, Plasmid, Polylysine, Receptor, Room temperature, Secr, Synthetic peptide, Synthetic peptides, Transfection, Transfection medium, Ziady.
Abstract
Background: The serpin–enzyme complex receptor (SECR) has previously been successfully targeted for gene delivery using synthetic peptide ligands covalently linked in fluid phase to commercially available polylysine preparations (∼10–54 kDa). The objective of the present study was to improve this approach by the use of small, bifunctional, and easily standardised synthetic peptides. Methods: Two synthetic peptides designated polylysine antitrypsin 1 (PAT1) (K16 FNKPFVFLI) and PAT2 (K16 CSIPPEVKFNKPFVFLI) were evaluated for gene delivery to the HUH7 human hepatocyte cell line. The K16 moiety binds DNA electrostatically, while the FVFLM motif of human α1‐antitrypsin targets the SECR. Results: Both PAT1 and PAT2 bind to and condense DNA into small particles as shown by laser scattering techniques. However, only PAT2 is effective for gene delivery, presumably on account of the greater distance between the K16 chain and the FVFLM motif. Gene delivery by PAT2/DNA complexes is chloroquine‐dependent, can be blocked completely by free ligand (CSIPPEVKFNKPFVFLI), and is highly efficient (e.g. approximately five‐fold more effective than lipofectamine). At physiological salt concentrations, PAT2/DNA complexes formed at 4 µg/ml DNA are ∼350 nm in diameter and highly effective for gene transfer, but at 100 µg/ml DNA the complexes are aggregated (diameter >4 µm) and inactive. Conclusions: A small (33 amino acid), bifunctional, synthetic peptide represents a highly efficient and readily standardised DNA vector for the SECR. The effectiveness of this peptide depends on the distance of the K16 moiety from the targeting ligand. High salt concentrations are not required to form effective vector/DNA complexes. Copyright © 2001 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/jgm.180
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000710
- to stream Istex, to step Curation: 000710
- to stream Istex, to step Checkpoint: 001247
- to stream Main, to step Merge: 002446
- to stream Main, to step Curation: 002421
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">A small, synthetic peptide for gene delivery via the serpin–enzyme complex receptor</title><author><name sortKey="Patel, Sheetal" sort="Patel, Sheetal" uniqKey="Patel S" first="Sheetal" last="Patel">Sheetal Patel</name></author><author><name sortKey="Zhang, Xiaohong" sort="Zhang, Xiaohong" uniqKey="Zhang X" first="Xiaohong" last="Zhang">Xiaohong Zhang</name></author><author><name sortKey="Collins, Louise" sort="Collins, Louise" uniqKey="Collins L" first="Louise" last="Collins">Louise Collins</name></author><author><name sortKey="Fabre, John W" sort="Fabre, John W" uniqKey="Fabre J" first="John W." last="Fabre">John W. Fabre</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:35AFB327C700AA45645D43A5593CAE81F45C50D5</idno><date when="2001" year="2001">2001</date><idno type="doi">10.1002/jgm.180</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-8KVCCXBG-T/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000710</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000710</idno><idno type="wicri:Area/Istex/Curation">000710</idno><idno type="wicri:Area/Istex/Checkpoint">001247</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001247</idno><idno type="wicri:doubleKey">1099-498X:2001:Patel S:a:small:synthetic</idno><idno type="wicri:Area/Main/Merge">002446</idno><idno type="wicri:Area/Main/Curation">002421</idno><idno type="wicri:Area/Main/Exploration">002421</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">A small, synthetic peptide for gene delivery via the serpin–enzyme complex receptor</title><author><name sortKey="Patel, Sheetal" sort="Patel, Sheetal" uniqKey="Patel S" first="Sheetal" last="Patel">Sheetal Patel</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Clinical Sciences, Institute of Liver Studies, Guy's, King's and St Thomas' School of Medicine, King's College Hospital, Bessemer Road, London SE5 9PJ</wicri:regionArea><wicri:noRegion>London SE5 9PJ</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Xiaohong" sort="Zhang, Xiaohong" uniqKey="Zhang X" first="Xiaohong" last="Zhang">Xiaohong Zhang</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Clinical Sciences, Institute of Liver Studies, Guy's, King's and St Thomas' School of Medicine, King's College Hospital, Bessemer Road, London SE5 9PJ</wicri:regionArea><wicri:noRegion>London SE5 9PJ</wicri:noRegion></affiliation></author><author><name sortKey="Collins, Louise" sort="Collins, Louise" uniqKey="Collins L" first="Louise" last="Collins">Louise Collins</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Clinical Sciences, Institute of Liver Studies, Guy's, King's and St Thomas' School of Medicine, King's College Hospital, Bessemer Road, London SE5 9PJ</wicri:regionArea><wicri:noRegion>London SE5 9PJ</wicri:noRegion></affiliation></author><author><name sortKey="Fabre, John W" sort="Fabre, John W" uniqKey="Fabre J" first="John W." last="Fabre">John W. Fabre</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Clinical Sciences, Institute of Liver Studies, Guy's, King's and St Thomas' School of Medicine, King's College Hospital, Bessemer Road, London SE5 9PJ</wicri:regionArea><wicri:noRegion>London SE5 9PJ</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Royaume-Uni</country></affiliation><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country><wicri:regionArea>Correspondence address: Department of Clinical Sciences, Institute of Liver Studies, King's College Hospital, Bessemer Road, London SE5 9PJ</wicri:regionArea><wicri:noRegion>London SE5 9PJ</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">The Journal of Gene Medicine</title><title level="j" type="alt">JOURNAL OF GENE MEDICINE, THE</title><idno type="ISSN">1099-498X</idno><idno type="eISSN">1521-2254</idno><imprint><biblScope unit="vol">3</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="271">271</biblScope><biblScope unit="page" to="279">279</biblScope><biblScope unit="page-count">9</biblScope><publisher>John Wiley & Sons, Ltd.</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2001-05">2001-05</date></imprint><idno type="ISSN">1099-498X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1099-498X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acid peptide</term><term>Amino acids</term><term>Average diameter</term><term>Bifunctional</term><term>Chloroquine</term><term>Complex receptor</term><term>Control peptide</term><term>Copyright</term><term>Culture medium</term><term>Dmem</term><term>Dmem medium</term><term>Effective complexes</term><term>Fvflm motif</term><term>Gene</term><term>Gene delivery</term><term>Gene transfer</term><term>Huh7</term><term>Huh7 cell line</term><term>Huh7 cells</term><term>Irrelevant peptide</term><term>Isotonic solutions</term><term>John wiley sons</term><term>Ligand</term><term>Lysine</term><term>Moiety</term><term>Nacl</term><term>Pat1</term><term>Pat1 peptide</term><term>Pat2</term><term>Pat2 peptide</term><term>Pat2 vector</term><term>Peptide</term><term>Pgl2</term><term>Pgl2 plasmid</term><term>Plasmid</term><term>Polylysine</term><term>Receptor</term><term>Room temperature</term><term>Secr</term><term>Synthetic peptide</term><term>Synthetic peptides</term><term>Transfection</term><term>Transfection medium</term><term>Ziady</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: The serpin–enzyme complex receptor (SECR) has previously been successfully targeted for gene delivery using synthetic peptide ligands covalently linked in fluid phase to commercially available polylysine preparations (∼10–54 kDa). The objective of the present study was to improve this approach by the use of small, bifunctional, and easily standardised synthetic peptides. Methods: Two synthetic peptides designated polylysine antitrypsin 1 (PAT1) (K16 FNKPFVFLI) and PAT2 (K16 CSIPPEVKFNKPFVFLI) were evaluated for gene delivery to the HUH7 human hepatocyte cell line. The K16 moiety binds DNA electrostatically, while the FVFLM motif of human α1‐antitrypsin targets the SECR. Results: Both PAT1 and PAT2 bind to and condense DNA into small particles as shown by laser scattering techniques. However, only PAT2 is effective for gene delivery, presumably on account of the greater distance between the K16 chain and the FVFLM motif. Gene delivery by PAT2/DNA complexes is chloroquine‐dependent, can be blocked completely by free ligand (CSIPPEVKFNKPFVFLI), and is highly efficient (e.g. approximately five‐fold more effective than lipofectamine). At physiological salt concentrations, PAT2/DNA complexes formed at 4 µg/ml DNA are ∼350 nm in diameter and highly effective for gene transfer, but at 100 µg/ml DNA the complexes are aggregated (diameter >4 µm) and inactive. Conclusions: A small (33 amino acid), bifunctional, synthetic peptide represents a highly efficient and readily standardised DNA vector for the SECR. The effectiveness of this peptide depends on the distance of the K16 moiety from the targeting ligand. High salt concentrations are not required to form effective vector/DNA complexes. Copyright © 2001 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Patel, Sheetal" sort="Patel, Sheetal" uniqKey="Patel S" first="Sheetal" last="Patel">Sheetal Patel</name></noRegion><name sortKey="Collins, Louise" sort="Collins, Louise" uniqKey="Collins L" first="Louise" last="Collins">Louise Collins</name><name sortKey="Fabre, John W" sort="Fabre, John W" uniqKey="Fabre J" first="John W." last="Fabre">John W. Fabre</name><name sortKey="Fabre, John W" sort="Fabre, John W" uniqKey="Fabre J" first="John W." last="Fabre">John W. Fabre</name><name sortKey="Fabre, John W" sort="Fabre, John W" uniqKey="Fabre J" first="John W." last="Fabre">John W. Fabre</name><name sortKey="Zhang, Xiaohong" sort="Zhang, Xiaohong" uniqKey="Zhang X" first="Xiaohong" last="Zhang">Xiaohong Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002421 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002421 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:35AFB327C700AA45645D43A5593CAE81F45C50D5
|texte= A small, synthetic peptide for gene delivery via the serpin–enzyme complex receptor
}}
| This area was generated with Dilib version V0.6.33. |  |